Emma Gunnell

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Attenuating the function of protein arginine methyltransferases PRMTs is an objective for the investigation and treatment of several diseases including cardiovascular disease and cancer. Bisubstrate inhibitors that simultaneously target binding sites for arginine substrate and the cofactor S-adenosylmethionine SAM have potential utility, but structural information on their binding is required for their development.

Moreover, a residue around the active site that differs between CARM1 Asn and PRMT1 Tyr is identified that affects the side chain conformation of the catalytically important neighbouring glutamate in the crystal structures. Mutagenesis data supports its contribution to the difference in binding observed for this inhibitor. Structures of CARM1 in complex with a range of seven inhibitors reveal the binding modes and show that inhibitors with an amino acid terminus adopt a single conformation whereas the electron density for equivalent amine-bearing inhibitors is consistent with preferential binding in two conformations.

Calculating singlet excited states: comparison with fast time-resolved infrared spectroscopy of coumarins Journal Article.

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Group by: Creators | Date | No Grouping. Jump to: | Gunnell, Emma () Investigating bisubstrate inhibitors of PRMT1 and CARM1. PhD thesis.

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